#Snapgene adaptors full#
L mature pollen stage, LP.02 two leaves visible stage, LP.04 four leaves visible stage, LP.06 six leaves visible stage, LP.08 eight leaves visible stage, LP.10 ten leaves visible stage, LP.12 twelve leaves visible stage, M germinated pollen stage, flowering stage, mature plant embryo stage, petal differentiation and expansion stage, plant embryo bilateral stage, plant embryo cotyledonary stage, plant embryo globular stage, vascular leaf senescent stageĬarpel, cauline leaf, collective leaf structure, cotyledon, cultured plant cell, flower, flower pedicel, guard cell, hypocotyl, inflorescence meristem, leaf apex, leaf lamina base, petal, petiole, plant embryo, plant sperm cell, pollen, pollen tube cell, root, rosette leaf, seed, sepal, shoot apex, shoot system, stamen, stem, vascular leaf, whole plantįull length CDS full length genomic full length cDNA protein Remove the heat block from the apparatus and allow to cool to room temperature (or at least below 30 C) on the workbench. each sample was ligated to one of 48 unique barcode adaptors. The recommended molar ratio of phosphorylated adaptor. incorporating the Semi-HMM based Nucleic Acid Parser (SNAP) gene predictor software, in. Place tube in a standard heatblock at 9095 C for 35 minutes. Conditions for adaptor ligation is the same as for insertion of DNA fragments into a plasmid vector. ADA3a and ADA3b Transcriptional Adaptors Regulate Gene Expression in Multiple Flowering.
Member of AtCBLs (Calcineurin B-like Calcium Sensor Proteins) interacts with CIPK3/9/23/26 resulting in phosphorylation of their downstream targets, influencing the maintenance of cellular magnesium homeostasis.ĭetection of calcium ion, potassium ion homeostasisĬytosol, membrane, plant-type vacuole, plant-type vacuole membrane, plasma membraneĬalcium ion binding, kinase binding, protein binding Annealing the Oligonucleotides : Mix equal volumes of both complementary oligos (at equimolar concentration) in a 1.5 ml microfuge tube. Primers were designed using the SnapGene software under default.
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